IJEP 42(9): 1088-1093 : Vol. 42 Issue. 9 (September 2022)
Abiyot Kelecha Geleta*, Megersa Abdisa, Milkessa Fenta and Birhanu Wakwoya
Mettu University, Department of Chemistry, College of Natural and Computational Science, Mettu, Ethiopia
Abstract
Coffee husk is considered as one of the agricultural waste that contains high amount of cellulose. Furthermore, the aim of this work was valourization of coffee husks to bioethanol by using separate hydrolysis and fermentation methods which are critically used as replacements for renewable sources of energy and to minimize environmental pollution. This method was accomplished by four major experimental procedures, namely pretreatment, hydrolysis, fermentation and distillation processes. A reflux method for hydrolysis, commercial yeast for fermentation, rotary vapour for distillation and high performance liquid chromatography with refractive index (HPLC-RI) for detection were used. The optimum hydrolysis and fermentation times were 4 hr and 48 hr, respectively. Under the same hydrolysis and fermentation times 5.72 g/L of ethanol was calculated for the lab-scale produced bioethanol and 7.1% for small scale done in locally made bioreactor. The limits of detection and quantitation calculated from the regression line were 0.0076% and 0.025%, respectively based on 3 and 10 times the standard deviation of the calibration plot.
Keywords
Coffee husk, Bioethanol, Hydrolysis, Small scale, Fermentation, Bioreactor
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