IJEP 44(13): 1176-1182 : Vol. 44 Issue. 13 (Conference 2024)
1. Graphic Era (Deemed to be University), Department of Microbiology, Dehradun – 248 002, Uttarakhand, India
2. Graphic Era (Deemed to be University), Department of Biotechnology, Dehradun – 248 002, Uttarakhand, India
Abstract
Myrica esculenta is a beneficial plant widely distributed in the Indian Himalayan region. It is classified in the family Myricaceae and is commonly known as Kaphal in Uttarakhand. Since ancient times, Himalayan plants have been an imminent source of food and therapeutic drugs for the local inhabitants as well as people living worldwide. The current study is framed with an aim to evaluate the development of distinct solvents, such as acetone, methanol and aqueous on the phenolic status while investigating the related antimicrobial response of distinct M. esculenta plant sections. In the current investigation, the crude extract of M. esculenta leaves, stems and barks were screened out for the presence of secondary metabolites and to evaluate the antimicrobial potential of the plant extracts against pathogenic microorganisms. The total flavonoid contents (TFC) and total phenolic contents (TPC) were calculated using the standard gallic acid and quercetin, respectively. The maximum TPC (1.60±0.01 mg/gm) and TFC (2.20±0.18 mg/gm) were observed in M. esculenta acetonic leaves extract and the minimum TPC (0.29±0.16 mg/gm) and TFC (0.22±0.07 mg/gm) were observed in M. esculenta aqueous leaves extract. Further, FTIR analysis was carried out to detect the occurrence of functional groups, such as alkenes (C=C), alkanes (C-H), carboxylic acids (C=O), nitro (N-O), sulphate (S=O), alcohols (O-H) and conjugated acid (C=O). These estimated bioactive compounds identified in different parts of M. esculenta extracts could be applied in various pharmaceutical, cosmetics, leather industries, etc. Furthermore, the antimicrobial activity of M. esculenta extracts were also tested against bacteria and fungi. M. esculenta acetonic leaf extract was found to be more potent against Staphylococcus aureus, showing a zone of inhibition of 7.4±0.2 mm in comparison to the other extracts of leaves, stem and bark. The determined efficacy of the plant extract can be a source of curative agent against health ailments.
Keywords
Myrica esculenta, Phytochemical screening, Phenolic, Flavonoids, Antimicrobial activity
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