IJEP 44(9): 801-808 : Vol. 44 Issue. 9 (September 2024)
1. Sri Ramakrishna College of Arts & Science, Department of Chemistry, Coimbatore – 641 044, Tamil Nadu, India
2. Annapoorana Engineering College, Department of Chemistry, Salem – 636 308, Tamil Nadu, India
3. KPR Institute of Engineering and Technology, Department of Chemistry, Coimbatore – 641 407, Tamil Nadu, India
Abstract
Biogenic synthesis of nickel oxide nanoparticles using Vitex negundo leaf extract has been reported in this current study because they are cost-effective and environment-friendly. Furthermore, the nickel oxide (NiO) nanoparticles prepared using green methods have better cytotoxicity and antibacterial activity. According to this paper, x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDAX) were used to characterize NiO nanoparticles. XRD and FTIR studies revealed the production of pure nickel oxide particles. The NiO nanoparticles that were produced were single crystalline with a face-centered cubic phase, respectively. Various doses of nickel oxide nanoparticles generated from Vitex negundo leaf extract, Eucalyptus leaf extract and a combination of the above extract were analysed for in-vitro cytotoxicity and cell survival of human cell cancer cell HT-29 (colon carcinoma cell lines) as well as antibacterial tests against various bacterial strains were studied. MTT cell viability measurements and morphological studies revealed that the synthesized NiO nanoparticles have anticancer activity when prepared in various zones against human cancer cells. Also, inhibition obtained revealed NiO nanoparticle’s effective antibacterial activity against various Gram-positive and Gram-negative bacterial pathogens.
Keywords
Biogenic synthesis, Nickel oxide nanoparticles, Eucalyptus leaf, Vitex negundo, Cytotoxicity, Antibacterial activity
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