Bioremediation potential of Bacillus amyloliquefaciens BAB-807 and Bacillus rugosus SPB7 Isolated from Deliming effluent

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IJEP 45(1): 3-15 : Vol. 45 Issue. 1 (January 2025)

Full Text

Raveena Jayam J. and K.J. Sharmila*

Dr. M.G.R. Educational and Research Institute, Department of Biotechnology, Maduravoyal, Chennai – 600 095, Tamil Nadu, India

Abstract

Heavy metal toxicity is rendered easy with bioremediation, an economical and environmentally friendly method. Five isolates were isolated from deliming effluent. The minimum inhibitory concentration (MICs) for each isolate varied in relation to the levels of the heavy metals upto 10,000 ppm. High-resistance isolates were identified by 16S rRNA as Bacillus amyloliquefaciens BAB-807OR740568 and Bacillus rugosus SPB7OR740574. The degradation capacity was assessed using atomic absorption spectroscopy (AAS). Bacillus amyloliquefaciens BAB-807 showed maximum degradation at 37oC at pH 9 for Cr (90%), Pb (90%), Ni (90.2%), Cu (91%) and Cd (80%); at 45oC, pH 9 for Cr (90%), Pb (92%), Ni (91.31%), Cu (93%) and Cd(81%) within 48 hr. However, at 55oC, pH 9, degradation observed was 96% (Cr), 93% (Pb), 92.31% (Ni), 93% (Cu) and 86% (Cd) in 48 hr. Bacillus rugosus SPB7 showed maximum degradation at 45oC, pH 9 in Cr (91%), Pb (92%), Ni (91.45%), Cu (92%) and Cd (78%). Furthermore, at 55oC at pH 9, degradation was 91% (Cr), 92% (Pb), 92% (Ni), 92% (Cu) and 78% (Cd) in 48 hr. This result conveys that the strength of Bacillus amyloliquefaciens BAB-807 and Bacillus rugosus SPB7 degradation is very effective at 370C, 45oC and 55oC temperatures for maximum exposure time. The findings of Bacillus amyloliquefaciens BAB-807and Bacillus rugosus SPB7 exhibiting various degrees of ability to degrade heavy metals potentially provide a basis for the advancement of bioremediation processes for the treatment of tannery effluent.

Keywords

Deliming effluent, Bacillus amyloliquefaciens BAB-807, Bacillus rugosus SPB7, Heavy metals, Bioremediation, Atomic absorption spectrometry

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